Direct Detection of Viable but Non-culturable (VBNC) Salmonella in Real Food System by a Rapid and Accurate PMA-CPA Technique
نویسندگان
چکیده
منابع مشابه
Differential Resuscitative Effect of Pyruvate and its Analogues on VBNC (Viable But Non-Culturable) Salmonella
An environmental isolate of Salmonella Enteritidis (SE), grown to the logarithmic phase, rapidly lost culturability by the addition of 3 mM H2O2 to cultures grown in Luria-Bertani (LB) medium; however, some H2O2-treated bacteria regained their culturability in M9 minimal medium, if sodium pyruvate was present at at least 0.3 mM. In addition, most pyruvate analogues, such as bromopyruvate or phe...
متن کاملCurrent Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria
Under stress conditions, many species of bacteria enter into starvation mode of metabolism or a physiologically viable but non-culturable (VBNC) state. Several human pathogenic bacteria have been reported to enter into the VBNC state under these conditions. The pathogenic VBNC bacteria cannot be grown using conventional culture media, although they continue to retain their viability and express...
متن کاملViable but Non-culturable Bacteria: Clinical Practice and Future Perspective
In this editorial, the importance of VBNC in clinical practice and future researches interests has been pinpointed. Accurate detection of VBNC, especially for major mentioned pathogens, is the future research gap.
متن کاملDetection of Viable But Non-Culturable State of Escherichia coli O157:H7 Using Reverse Transcription PCR
Background and Aims: Many bacteria including Escherichia coli may enter into a viable but non-culturable (VBNC) state under unfavorable stresses, which are unable to be detected by culture-based methods. In this study, the use of Reverse Transcription PCR (RT-PCR) for detection of VBNC state of E. coli O157:H7 was investigated. Materials and Methods: Escherichia. coli O157:H7 was inoculated i...
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Salmonella paratyphi B and Salmonella typhimurium were starved in seawater microcosms respectively for 12 and 6 years. After starvation periods, major part of water was evaporated in each microcosm and salt crystals appeared. No culturable cells were obtained in selective and non-selective media. Salty suspension of each microcosm was inoculated in the yolk sacs of embryonated chicken eggs. Cul...
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2021
ISSN: 1664-302X
DOI: 10.3389/fmicb.2021.634555